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郭萌萌.转双抗虫基因107杨节肢动物群落特征及相对稳定性.生态学报,2018,(1).http://dx.doi.org/10.5846/stxb201612202623  
转双抗虫基因107杨节肢动物群落特征及相对稳定性
Arthropod community characteristics and relative stability in transgenic Populus × euramericana ‘Neva’ carrying bivalent insect-resistant genes
投稿时间:2016-12-20  修订日期:2017-07-28
DOI: 10.5846/stxb201612202623
关键词转抗虫基因107杨  群落特征  相对稳定性  主成分分析
Key Wordstransgenic insect-resistant 107 poplar  community characteristics  relative stability  principal component analysis
基金项目国家高技术研究发展计划(863计划);国家林业局科技发展中心项目;国家自然科学基金项目;内蒙古自治区自然科学基金项目
作者单位E-mail
郭萌萌 河北农业大学 1103083489@qq.com 
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摘要:
于2015年5—9月对唐山市滦南县苗圃场内的转双抗虫基因(BtCry1Ac和API基因)107杨(简称:转抗虫基因107杨)及未转基因107杨(简称:对照杨)进行了节肢动物群落调查,利用群落特征指数、群落相似性系数、群落相对稳定性和主成分分析法对转抗虫基因107杨节肢动物群落特征、相似性、相对稳定性及主成分进行对比分析。调查共获得节肢动物6818头,隶属于2纲,8目,43科,58种。研究结果表明:转抗虫基因107杨和对照杨节肢动物群落中以鳞翅目、鞘翅目、膜翅目、双翅目昆虫为主要类群,其中鳞翅目昆虫个体数量最多;鳞翅目和半翅目昆虫个体数量与对照杨差异显著;在功能类群上,转抗虫基因107杨食叶昆虫个体数量较对照杨显著减少,刺吸昆虫个体数量显著增加;转抗虫基因107杨节肢动物群落多样性指数、均匀度指数较高,优势度指数较低,节肢动物群落物种分布较均匀;相似性结果显示,物种组成与对照杨相似度较高;转抗虫基因107杨节肢动物群落物种间在数量上的制约作用较强;主成分分析表明食叶昆虫物种数量与个体数量、刺吸昆虫物种数量和其它植食性昆虫个体数量是影响转抗虫基因107杨与对照杨节肢动物群落变化的共同主导因子。
Abstract:
Abstract: Genetically modified Bacillus thuringiensis is widely used for insect resistance. With the large-scale commercial application of transgenic insect-resistant plants, the risk of environmental release is a concern. The development of transgenic poplars could effectively control poplar pests, and the fast growth of the species makes it suitable for road greening applications. Hence, it is necessary to research and breed transgenic insect-resistant species with socioeconomic benefits. Transgenic Populus × euramericana ‘Neva’ carrying bivalent insect-resistant genes (transgenic insect-resistant 107 poplar) are mainly used via the agrobacterium-mediated transformation method for gene transformation research in Populus × euramericana ‘Neva’ using bi-insect-resistant genes built in a vector (BtCry1Ac gene API arrowhead protease inhibitor gene). For transgenic plants, molecular detection and insect feeding trials have been carried out, resulting in the development of transgenic insect-resistant 107 poplar strains. The transgenic insect-resistant 107 poplar has perennial characteristics; it may directly or indirectly affect the ecological system where planted in the field on the large-scale. To study the effect of transgenic insect-resistant 107 poplar on arthropod community characteristics and relative stability, the arthropod communities in forests were monitored and analyzed. The research aimed to provide data support for the ecological security evaluation of transgenic poplar, and provide a reference for the popularization and application of transgenic 107 poplar. We investigated the arthropod community in transgenic insect-resistant 107 poplars in a nursery field in Luannan County, Tangshan City in 2015, using the community characteristic index, community similarity, community relative stability, and principal component analysis. In total, 6,818 insects, belonging to 2 classes, 8 orders, 43 families, and 58 species, were collected. The results showed that Lepidoptera, Coleoptera, Hymenoptera, and Diptera were the dominant species of transgenic insect-resistant 107 poplars, and Lepidoptera were the most abundant. The numbers of Lepidopteran and hemipteran individuals were significantly different between transgenic insect-resistant 107 poplars and the control. Compared with numbers in the control, the number of individuals of herbivorous insects in transgenic insect-resistant 107 poplars were significantly lower, and the number of the individuals of sucking insects were significantly higher. Compared with the control for the community characteristic indexes, the arthropod community diversity index and evenness index of transgenic insect-resistant 107 poplars were higher, whereas the community dominance index was lower. These results show that the species distribution of the arthropod community was more uniform in transgenic insect resistant 107 poplars than in the control. The analysis of community similarity shows that the similarity of species composition between the transgenic insect-resistant 107 poplar was higher than that of the control. Analysis of the relative stability of the community showed that the number of arthropod communities in transgenic insect-resistant 107 poplars was stronger than that of the control. The number of species and individuals of different functional groups of arthropod communities were more affected by the transgenic insect-resistant 107 poplar than by the control.The main factors affecting the change of arthropod communities in transgenic insect-resistant 107 poplars and the control were the herbivorous insect species and individual numbers, the number of sucking insects, and other species of phytophagous insects.
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