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刘海洋,姚举,张仁福,王伟,余璇,王琦.黄萎病不同发生程度棉田中土壤微生物多样性.生态学报,2018,(5).http://dx.doi.org/10.5846/stxb201702060218  
黄萎病不同发生程度棉田中土壤微生物多样性
Analysis of soil microbial diversity in cotton fields differing in occurrence of cotton Verticillium wilt in Xinjiang
投稿时间:2017-02-06  修订日期:2017-10-16
DOI: 10.5846/stxb201702060218
关键词根际土壤微生物  棉花黄萎病  高通量测序  土壤肥力
Key Wordsrhizosphere soil microorganism  cotton verticillium wilt  high-throughput sequencing  soil fertility
基金项目新疆自治区重点研发计划项目(2016B01001-6);国家棉花产业技术体系岗位科学家项目(CARS-18);公益性行业(农业)科研专项经费项目(201503112-6) *
作者单位E-mail
刘海洋 中国农业大学植物保护学院新疆农业科学院植物保护研究所 liuhaiyang001@163.com 
姚举 新疆农业科学院植物保护研究所  
张仁福 新疆农业科学院植物保护研究所  
王伟 新疆农业科学院植物保护研究所  
余璇 新疆农业大学农学院  
王琦 中国农业大学植物保护学院 wangqi@cau.edu.cn 
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摘要:
作物根际土壤微生物群落对土壤生态及作物健康至关重要。以棉花黄萎病不同发生程度棉田的土壤为研究对象,采用理化分析、微生物纯培养及高通量测序技术对土壤理化性质及微生物数量、细菌丰度多样性进行综合分析。结果表明:在纯培养条件下,大丽轮枝菌无菌发酵滤液对细菌生长有明显的抑制作用;棉田接种大丽轮枝菌对土壤中细菌、真菌、放线菌的数量及细菌菌群丰度多样性未产生明显影响,不同采样时间的土壤中细菌菌群结构差异更大。土壤中大丽轮枝菌微菌核数量与棉花黄萎病的发生程度呈显著正相关。土壤肥力对土壤中微生物数量起主导作用,而水稻—棉花轮作能够使棉田有效降盐、减病、改善土壤肥力。通过生物防治、作物轮作、深翻等调控措施增加土壤中有益菌群数量、改善土壤生态、降低棉田土壤中大丽轮枝菌菌源数量是减轻棉花黄萎病危害的基础。
Abstract:
Soil microbial community composition of crop rhizosphere is very important in soil ecology and crop health. In this paper, the soil with different levels of cotton Verticillium wilt is the research object. Moreover, physical and chemical properties of soil, and rhizosphere microbial community diversity were analyzed with the technique of microbial pure culture, physical and chemical analysis and high-throughput sequencing . The results indicate that sterile fermentation liquor of Verticillium dahliae has obvious inhibitory effect on bacterial growth in pure culture conditions. After inoculation with V. dahliae, the quantity of bacteria, fungi, and actinomycetes in the cotton soil show little change compared to that in the control. High-throughput sequencing analysis indicates that bacterial genetic relationship in soil shows no significant change, and sampling time has a bigger impact on the bacterial population structure. Soil fertility plays a key role in determining the quantity of bacteria, fungi, and actinomycetes in continuous cotton cropping soil, in which Verticillium wilt occurrence is severe and significantly higher than that in the control field. Rice-cotton rotation cropping has a strong effect on reducing salt, plant diseases, and improving soil fertility. Microsclerotia content in soil has significant positive correlation with the incidence of cotton Verticillium wilt. Soil fertility, culture measures, and crop varieties mainly influence soil microorganism quantity; however, Verticillium wilt occurrence does not cause significant change in microbial quantity. Reducing the quantity of microsclerotia in soil is the fundamental method of controlling Verticillium wilt.
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