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孙帅,张小晶,刘金平,游明鸿,郭碧花.遮阴和干旱对荩草生理代谢及抗性系统影响的协同作用.生态学报,2018,(5).http://dx.doi.org/10.5846/stxb201702160259  
遮阴和干旱对荩草生理代谢及抗性系统影响的协同作用
Synergy Effect of Shade and Drought on Physiological Metabolism and Resistance System of Arthraxon hispidus
投稿时间:2017-02-16  修订日期:2017-10-17
DOI: 10.5846/stxb201702160259
关键词光合作用  新陈代谢  抗氧化酶  协同作用  生长策略
Key WordsPhotosynthesis, Metabolism, Antioxidant  enzymes, Synergistic  effect, Growth  strategy
基金项目四川省科技支撑计划(2011NZ0064)项目;西华师范大学国家一般培育(17C046)项目
作者单位E-mail
孙帅 西华师范大学 jpgg2000@163.com 
张小晶 西华师范大学西南野生动植物资源保护教育部重点实验室  
刘金平 西华师范大学西南野生动植物资源保护教育部重点实验室;四川省草原科学研究院 jpgg2000@163.com 
游明鸿 四川省草原科学研究院  
郭碧花 西华师范大学  
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摘要:
以具有边坡草坪开发价值的野生荩草为材料,设置5个遮阴梯度和4个干旱梯度交互形成的20个协同处理,通过测定光合速率(Pn)、蒸腾速率(Tr)、胞间CO2浓度(Ci)、气孔速度(Gs)等光合作用参数,超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性等抗氧化酶系统参数,可溶性糖(SS)、丙酮酸含量(PA)和硝酸还原酶(NR)活性等糖代谢和氮素代谢及呼吸作用参数,丙二醛(MDA)、脯氨酸(Pro)、可溶性蛋白(SP)和过氧化氢(H2O2)含量等细胞膜伤害程度参数,分析遮阴和干旱对荩草生理生化及抗性系统影响的协同作用。结果表明:遮阴、干旱和协同作用对光合生理有显著影响(P<0.05),遮阴为影响Pn的主要因子,干旱为影响Gs、Ci 和Tr的主要因子,≤30%遮阴可缓解干旱引起Pn、Tr、Ci、Gs下降程度,遮阴和干旱对光合参数的协同作用顺序为Tr>Ci>Pn>Gs;遮阴、干旱和协同作用对糖代谢(SS)、呼吸作用(PA)和氮代谢(NR)有显著影响(P<0.05),干旱是影响代谢的主要因子,协同作用次之,遮阴影响较小,遮阴度越大SS含量越低、PA含量越大,干旱越重SS和PA含量越大,NR活性受干旱或遮阴胁迫影响最大,协同作用影响大小为PA>NR>SS。遮阴、干旱和协同作用对抗氧化酶系统有显著影响(P<0.05),随遮阴度或干旱度增加抗性指标先显著增加又显著降低,干旱是影响抗性指标主要因子,遮阴次之,协同作用较小,10-30%遮阴提高了中轻度干旱下抗性指标和重度干旱下SOD和POD活性,遮阴和干旱对抗氧化酶显著协同影响的大小为CAT>SOD>POD。遮阴、干旱和协同作用对细胞膜伤害指标有显著影响(P<0.05),干旱对H2O2、Pro和SP的影响大于遮阴,遮阴对MDA影响大于干旱,遮阴和干旱对SP无协同影响,对Pro有显著协同作用(P<0.05),对H2O2和MDA有极显著协同影响(P<0.01)。遮阴和干旱协同作用提高了荩草的光合能力、代谢速率、抗逆潜力和生态适应范围。
Abstract:
In the study, wild Arthraxon hispidus, a slope lawn development potential plant, was used as test materials, and was set up 20 cooperative teatments with five shade gradients (0、10%、30%、50%、70%) and four drought gradient (enough moisture (W0), mild drought (W1), moderate drought (W2), severe drought (W3)). After treating 36 days, some index including photosynthetic parameters(photosynthetic rate (Pn), transpiration rate (Tr), intercellular CO2 concentration (Ci), porosity (Gs), etc.), antioxidant enzyme system parameters(superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) , etc.) , parameters associated with metabolism(soluble sugar (SS), acetone acid (PA) and nitrate reductase (NR) activity, and parameters associated with cell membrane damage(malondialdehyde (MDA) and proline (Pro), soluble protein (SP) and hydrogen peroxide (H2O2) were determined. The purpose was to analyze synergy effect of shade and drought on physiological, biochemical and resistance system of A. hispidus. The results showed that: Shade, drought and their synergy had a significant effect on photosynthetic physiology (P< 0.05). Shade was main factor affecting Pn, and drought was main factor affecting Gs, Ci and Tr. 30% or less shade could alleviate the dropping degree of Pn, Tr, Ci and Gs caused by drought. The order of their synergitical effect on photosynthetic parameters was Tr > Ci > Pn > Gs. Shade, drought and synergy also had a significant effect on SS, PA, and NR (P < 0.05). Drought was main factor affecting metabolism, follwed by synergy and shade in turn. The more shade degree was, the less SS content and the bigger PA content was. The heavier drought was, the more SS and PA content were.NR activity was most affected by drought and shading stress. The order of synergitical effect was PA > NR > SS. Shade, drought and their synergy also had a significant effect on antioxidant enzyme system (P < 0.05). With shading or drought increasing, resistance index significantly increased, then significantly decreased. Drought was main factor affecting resistance index, followed by shading and synergy in turn. 10-30% shading increased resistance index in the moderate drought and SOD and POD acvitity in the severe drought. The order of synergitical effect on antioxidant enzymes was CAT>SOD>POD. Shade, drought and synergy also had a significant effect on membrane damage index(P<0.05). Drought had a bigger effect on H2O2, Pro and SP than shading, yet shading had a bigger effect on MDA than drought. Shade and drought had no synergistic effect on SP, and a significant synergistic effect on Pro(P<0.05), and a high significant effect on H2O2 and MDA(P<0.01). Therefore, synergetic effects of shading and drought improved photosynthetic capacity, metabolic rate and resistance potential of H. hispidus, which expanded ecological adaptability of H. hispidus.
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