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杨淑娇,杨永平,石玲玲,毕迎凤,李建文,杨锦超,杨雪飞.基于PLFA研究高山栎和高山松林的松茸菌塘土壤微生物群落特征.生态学报,2018,(5).http://dx.doi.org/10.5846/stxb201701200169  
基于PLFA研究高山栎和高山松林的松茸菌塘土壤微生物群落特征
Comparison of soil microbial community composition and structure in Tricholoma matsutake shiro and non-shiro soils in Quercus semecarpifolia and Pinus densata forests
投稿时间:2017-01-20  修订日期:2017-10-18
DOI: 10.5846/stxb201701200169
关键词松茸  菌塘  土壤微生物群落  林型  磷脂脂肪酸(PLFA)
Key WordsTricholoma matsutake  shiro  soil microbial community  forest type  phospholipid fatty acid (PLFA)
基金项目国家自然科学基金项目(31370513);中国科学院“西部之光”人才培养引进计划
作者单位E-mail
杨淑娇 中国科学院昆明植物研究所 yangshujiao@mail.kib.ac.cn 
杨永平 中国科学院昆明植物研究所 yangyp@mail.kib.ac.cn 
石玲玲 中国科学院昆明植物研究所 shilingling@mail.kib.ac.cn 
毕迎凤 中国科学院昆明植物研究所 biyingfeng@mail.kib.ac.cn 
李建文 中国科学院昆明植物研究所 lijianwen@mail.kib.ac.cn 
杨锦超 中国科学院昆明植物研究所 469973519@qq.com 
杨雪飞 中国科学院昆明植物研究所 xuefei@mail.kib.ac.cn 
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摘要:
松茸菌塘内的土壤微生物群落组成和结构特征是否与非菌塘存在差异,差异是否随宿主森林类型而改变?通过磷脂脂肪酸(Phospholipid fatty acid,PLFA)生物标记法对云南省香格里拉县的高山栎林和高山松林内的松茸菌塘和非菌塘的土壤微生物群落进行对比分析。结果显示:1)菌塘内的优势菌群主要包括真菌(18:2ω6,9c、18:1ω9c、18:3 w6c(6,9,12))、革兰氏阴性菌(19:0 cyclo w8c)、革兰氏阳性菌(15:0 iso)和未被鉴定的PLFAs(16:0、17:1 anteiso B、18:1 w6c、16:1 w6c和18:0);2)从群落组成上看,菌塘内的真菌百分含量、真菌/细菌生物量比(F/B)显著高于非菌塘,而革兰氏阴性菌(GNB)和革兰氏阳性菌(GPB)的百分含量,以及总PLFAs均匀度(J)均低于非菌塘;3)从群落结构上看,土壤微生物群落在菌塘和非菌塘间、以及不同林型间均存在显著差异,且菌塘对群落结构的影响大于林型;4)无论是在群落组成还是群落结构上,林型均不与菌塘存在交互作用,即松茸菌塘的土壤微生物群落特征不随宿主森林类型而发生改变;5)土壤化学性质与林型密切相关,但与菌塘关系不明显。上述结果表明,菌塘内的土壤微生物群落组成和结构与非菌塘有所不同,且在两种林型中表现相似。
Abstract:
The composition and structure of the soil microbial community is a key determinant of the growth and development of the prized edible mycorrhizal fungus Tricholoma matsutake and its shiro. However, little is known about any differences in the composition and structure of soil microbial communities between shiro and non-shiro soils, and whether these differences vary among different host forest types. In this study, we used phospholipid fatty acid (PLFA) analysis to compare the soil microbial communities of shiro and non-shiro soils in two forests dominated by Quercus semecarpifolia and Pinus densata in Shangri-La County in northwest Yunnan, China. We found that: 1) fungi (18:2ω6,9c, 18:1ω9c, 18:3 w6c (6,9,12)), gram-negative bacteria (19:0 cyclo w8c), gram-positive bacteria (15:0 iso), and non-specific PLFAs (16:0, 17:1 anteiso B, 18:1 w6c, 16:1 w6c, 18:0) were the dominant microbial groups in shiro; 2) the percentage of fungi and the biomass ratio of fungi to bacteria (F/B) were significantly higher in shiro than in non-shiro soils, but the percentages of gram-negative bacteria and gram-positive bacteria, and the evenness (J) of total PLFAs were lower in shiro than in non-shiro soils; 3) soil microbial community structure differed significantly between shiro and non-shiro soils and between the two forest types, and the difference between shiro and non-shiro soils was greater than the difference between the two forest types; 4) there was no interaction between shiro and forest type, which indicates that shiro microbial community characteristics did not differ between host forest types; 5) soil chemical properties differed significantly between the two forest types; however, no obvious difference was found between shiro and non-shiro soils. Our findings revealed differences in the composition and structure of shiro and non-shiro soil microbial communities , and that these differences were consistent across both Quercus semecarpifolia and Pinus densata forests.
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