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李欣玫,左易灵,薛子可,赵丽莉,贺学礼.不同荒漠植物根际土壤微生物群落结构特征.生态学报,2018,(8).http://dx.doi.org/10.5846/stxb201704210722  
不同荒漠植物根际土壤微生物群落结构特征
Characteristics of soil microbial community structure in the rhizosphere of different desert plants
投稿时间:2017-04-21  修订日期:2017-12-04
DOI: 10.5846/stxb201704210722
关键词土壤微生物群落  磷脂脂肪酸(PLFA)  荒漠植物  甘肃安西荒漠
Key WordsSoil microbial community  phospholipid fatty acid (PLFA)  desert plants  Anxi extremely arid desert in Gansu
基金项目国家自然科学基金项目(31470533, 31770561)
作者单位E-mail
李欣玫 河北大学生命科学学院 632348667@qq.com 
左易灵 河北大学生命科学学院  
薛子可 河北大学生命科学学院  
赵丽莉 河北大学生命科学学院  
贺学礼 河北大学生命科学学院 xuelh1256@aliyun.com 
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摘要:
于2015年7月在甘肃安西极旱荒漠国家级自然保护区采集膜果麻黄(Ephedra przewalskii)、红砂(Reaumuria songarica)、合头草(Sympegma regelii)、泡泡刺(Nitraria sphaerocarpa)和珍珠猪毛菜(Salsola passerina)5种典型荒漠植物根际土壤样品,利用磷脂脂肪酸(PLFAs)法结合Sherlock微生物鉴定系统分析了土壤微生物群落结构。结果表明,5种荒漠植物根际土壤微生物磷脂脂肪酸种类和组成差异显著,其中表征革兰氏阳性菌的18:0 iso、16:0iso分别为红砂、珍珠猪毛菜所特有,表征革兰氏阴性菌的17:1 iso ω9c和表征放线菌的18:1 ω7c 10-methyl仅在珍珠猪毛菜根际存在。总PLFAs、真菌、放线菌和真菌/细菌在珍珠猪毛菜中显著最高,革兰氏阳性菌、革兰氏阴性菌在膜果麻黄和珍珠猪毛菜中显著高于其它植物,AM真菌在合头草中有最高值。结构方程模型分析表明,与植物相比,土壤因子对微生物群落结构影响更为显著,其中易提取球囊霉素是革兰氏阳性菌、革兰氏阴性菌和放线菌主要影响因子,土壤全氮对AM真菌有显著影响,说明土壤全氮和易提取球囊霉素是影响土壤微生物PLFA变化的重要因子。同时,土壤微生物群落结构可用于检测不同荒漠植物根际微环境土壤退化状况。
Abstract:
In order to reveal the influence of different desert plants and soil factors on soil microbial community structure, phospholipid fatty acid (PLFAs) method combined with Sherlock microbial identification system was used to analyze the soil microbial community structure. The rhizosphere soil samples were collected from 5 kinds of typical desert plants, such as Ephedra przewalskii, Reaumuria songarica, Sympegma regelii, Nitraria sphaerocarpa and Salsola passerine in Anxi Extremely Arid Desert National Nature Reserve in Gansu province, in July 2015. The results showed that there were significant differences in soil microbial phospholipid fatty acid species and composition among 5 kinds of desert plants. The phospholipid fatty acid 18:0 iso and 16:0 iso, indicating Gram positive bacteria, were only detected in R. songarica and S. passerine, respectively. The phospholipid fatty acid 17:1 iso ω9c and 18:1 ω7c 10-methyl, representing gram-negative bacteria and actinomycetes, respectively, only existed in S. passerine. Total PLFAs, fungi, actinomycetes and fungi/bacteria under S. passerine were the highest, Gram-positive bacteria and gram-negative bacteria were significantly higher under E. przewalskii and S. passerine than under other plants, and there was a peak value of AM fungi under S. regelii. The Structural equation model analysis indicated that the influence of soil factors on soil microbial community structure was more significant than that of plant species. Easily extracted glomalin related soil protein was the main factor of gram positive bacteria, actinomyces and gram-negative bacteria, and total nitrogen had a significant influence on AM fungi, indicating the two soil variables were the important factors influencing soil microbial community structure. Moreover, soil microbial community structure can be used to monitor the soil degradation of the rhizospheric microenvironment of different desert plants.
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