首页关于本刊影响因子及获奖投稿须知订阅及广告专辑与专题学术会议绿色发表通道优秀论文 
郭建国,赵龙浩,徐丹,孙野青.人工湿地处理造纸废水后细菌群落结构变化.生态学报,2014,34(8):2095~2101 本文二维码信息
二维码(扫一下试试看!)
人工湿地处理造纸废水后细菌群落结构变化
The bacterial community changes after papermaking wastewater treatment with constructed wetlands
投稿时间:2013-07-01  修订日期:2014-03-26
DOI: 10.5846/stxb201307011813
关键词人工湿地  造纸废水  细菌群落
Key Wordsconstructed wetland  papermaking wastewater  bacterial community
基金项目国家科技支撑项目(2010BAC68B02);大连市科技计划项目(2011E11SF007)
作者单位E-mail
郭建国 大连海事大学环境系统生物学研究所, 大连 116026  
赵龙浩 大连海事大学环境系统生物学研究所, 大连 116026  
徐丹 大连海事大学环境系统生物学研究所, 大连 116026  
孙野青 大连海事大学环境系统生物学研究所, 大连 116026 yqsun@dlmu.edu.cn 
摘要点击次数 1127
全文下载次数 624
摘要:
人工湿地通过模拟自然湿地的生态系统,能够实现污染物的去除,是一种高效经济的污水处理手段,但是对人工湿地处理后污水中细菌群落的研究较少。采集了造纸厂排污口和人工湿地出水口的水样,检测了溶解氧、pH值和盐度等水质指标,对主要污染物的含量进行了测定和分析,并利用DGGE技术对细菌群落的变化进行了研究。结果表明:(1)造纸污水经人工湿地处理后水质有明显提高;(2)人工湿地处理后细菌群落结构发生变化,优势菌由γ变形菌和衣原体变为α变形菌,而且微生物多样性指数Shannon-Wiener’s降低;(3)人工湿地处理前污水中存在着大量致病微生物和降解微生物,处理后以环境友好的固氮菌和少量致病菌为主。研究结果揭示了人工湿地不仅可以去除造纸废水中的污染物,改善水质而且可以大大减少向环境中排放的致病微生物,防止由致病微生物引起的生态灾难的发生,为将来人工湿地应用于工业污水处理和微生物生态安全评估提供有效可靠的依据。
Abstract:
Constructed wetlands are efficient and economic sewage disposal means, characterized by stimulation of natural wetland ecosystems. They could remove the pollutants of wastewater to achieve purification of sewage, and microbes play an important role in this process. The aim of this study is to investigate bacterial community structure variation after effective wastewater treatment with constructed wetlands. We collected the water samples from the outfall of paper mill and outlet of constructed wetlands, and examined several parameters of the conventional water quality. Using DGGE method and cloning sequencing analysis of dominant microbes, we analyzed the variation of the bacterial community structure and major microbial species in the water samples. The results are as follows: (1)The water quality was greatly improved after papermaking wastewater treatment with constructed wetlands; (2) The structure of bacterial community remarkably changed, showing that the major microbes in the wastewater changed from Gamma-proteobacteria and Chlamydia to Alpha-proteobacteria, with the decrease in the Shannon-wiener's index; (3)Before papermaking wastewater treatment, dominant pathogenic bacteria included Legionella, Klebsiella and Parachlamydia and degrading bacteria included Thioalkalivibrio, Dehalococcoides and Hyphomicrobium. In contrast, the main priority of microbes became environment friendly bacteria such as Mesorhizobium and Rhizobium mostly, and the species and proportion of pathogenic bacteria significantly decreased after treatment with constructed wetlands. These findings suggest that constructed wetlands can not only improve water quality and change the structure of microbial community, but also remove a great deal of pathogens in the wastewater, providing powerful experimental evidence for application of constructed wetlands in industrial wastewater treatment and assessment of microbial ecology safety in the future.
HTML 查看全文   查看/发表评论  下载PDF阅读器

您是本站第 67403762 位访问者

Copyright © 2005-2019   京ICP备06018880号
地址:北京海淀区双清路18号
  邮编:100085    电话:010-62941099
  E-mail : shengtaixuebao@rcees.ac.cn
本系统由北京勤云科技发展有限公司提供技术支持