首页关于本刊影响因子及获奖投稿须知订阅及广告专辑与专题学术会议绿色发表通道优秀论文 
姜瑛,吴越,徐莉,胡锋,李辉信.悉生培养微缩体系食细菌线虫提高土壤激素含量的机制.生态学报,2016,36(9):2528~2536 本文二维码信息
二维码(扫一下试试看!)
悉生培养微缩体系食细菌线虫提高土壤激素含量的机制
The possible mechanisms underlying improvement of soil auxin content by bacterial-feeding nematodes in a gnotobiotic microcosm experiment
投稿时间:2014-11-10  修订日期:2016-02-01
DOI: 10.5846/stxb201411102223
关键词食细菌线虫  产激素菌  悉生培养  相互作用  IAA(吲哚乙酸)
Key Wordsbacterial-feeding nematodes  IAA-producing bacteria  gnotobiotic microcosm experiment  interaction  IAA(indole acetic acid)
基金项目国家自然科学基金项目(30970537,41401274)
作者单位E-mail
姜瑛 南京农业大学资源与环境科学学院, 南京 210095;河南农业大学资源与环境学院, 郑州 450002  
吴越 南京农业大学资源与环境科学学院, 南京 210095;山东省土壤肥料总站, 济南 250100  
徐莉 南京农业大学资源与环境科学学院, 南京 210095  
胡锋 南京农业大学资源与环境科学学院, 南京 210095  
李辉信 南京农业大学资源与环境科学学院, 南京 210095 huixinli@njau.edu.cn 
摘要点击次数 568
全文下载次数 437
摘要:
研究土壤食细菌线虫与细菌的相互作用及其生态功能是土壤生态学的核心内容之一。食细菌线虫取食细菌可以促进土壤中氮素的矿化,提高氮素养分的供给,改善土壤的营养条件,从而促进植物的生长发育。土壤食细菌线虫促进植物根系生长的"养分作用机制"已得到确认,而"激素作用机制"还存在争议。从供试土壤中筛选获得一株高效产IAA细菌和两种不同cp值的食细菌线虫,通过设置简化的悉生培养系统,对这两种土著食细菌线虫与土著产IAA细菌之间的相互作用,及其对土壤中IAA含量变化的影响进行研究。结果表明:两种食细菌线虫的取食均能促进细菌数量和活性的增强,食细菌线虫与产IAA细菌相互作用也能显著增加土壤中IAA的含量;这些促进作用受到接种食细菌线虫的种类以及培养时间的影响:在培养第10天和第20天时,接种cp值为1的中杆属食细菌线虫显著增加了产IAA细菌的数量;在培养第10天和第30天时,相比较接种cp值为2的头叶属食细菌线虫,接种中杆属食细菌线虫显著提高了土壤中IAA的含量。
Abstract:
In recent years, the interactions between bacterial-feeding nematodes and bacteria and their ecological functions in terrestrial ecosystems have been recognized to play a crucial role in soil ecology. Many studies have found that bacterial-feeding nematodes increased soil mineralization and improved the supply of inorganic nitrogen, subsequentlystimulating plant growth. The nutrient effect of the soil bacterial-feeding nematodes on root development has been investigated in a microcosm, while the auxin effect remains controversal. An indole acetic acid (IAA)-producing bacteria and two species of bacterial-feeding nematodes were isolated, screened and identified from our experimental soil. Then, a study on the effect of the interaction between aboriginal bacterial-feeding nematodes and IAA-producing bacteria on soil IAA content was implemented in a gnotobiotic microcosm experiment. In the gnotobiotic microcosm experiment, two species of aboriginal nematodes (Cephalobus sp. and Mesorhabditis sp.) grazing on bacteria stimulated both the bacterial growth and activity and increased the mineral nitrogen. The interactions between IAA-producing bacteria and bacterial-feeding nematodes significantly increased soil IAA content. This increase was influenced by bacterial-feeding nematode species and incubation time. Mesorhabditis sp. (cp 1) significantly stimulated the growth of the IAA-producing bacteria at day 10 and day 20. However, compared with Cephalobus sp. (cp 2), Mesorhabditis sp. more significantly increased soil IAA content at day 10 and day 30.
HTML 查看全文   查看/发表评论  下载PDF阅读器

您是本站第 65581625 位访问者

Copyright © 2005-2019   京ICP备06018880号
地址:北京海淀区双清路18号
  邮编:100085    电话:010-62941099
  E-mail : shengtaixuebao@rcees.ac.cn
本系统由北京勤云科技发展有限公司提供技术支持