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刘姣,刘长发,李盛德,李晋,陶韦,李璐瑶,马悦欣.翅碱蓬对盐沼沉积物微生物生物量及β-氨氧化细菌群落的影响——以双台河口为例.生态学报,2016,36(24):8081~8090 本文二维码信息
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翅碱蓬对盐沼沉积物微生物生物量及β-氨氧化细菌群落的影响——以双台河口为例
Effects of Suaeda heteroptera on microbial biomass and the community structure of β-ammonia-oxidizing bacteria in salt marsh sediments: The case of the Shuangtai estuary
投稿时间:2015-05-07  最后修改时间:2016-10-14
DOI: 10.5846/stxb201505070946
关键词翅碱蓬  微生物生物量  16S rRNA基因丰度  硝化作用  β-AOB
Key WordsSuaeda heteroptera  microbial biomass  16S rRNA gene abundance  nitrification  β-AOB
基金项目国家自然科学基金资助项目(41171389);国家海洋公益性行业科研专项资助项目(201305043);辽宁省高等学校优秀科技人才支持计划资助项目(LR2013035)
作者单位E-mail
刘姣 农业部海洋水产增养殖学重点开放实验室, 大连 116023  
刘长发 辽宁省高校近岸海洋环境科学与技术重点实验室, 大连 116023  
李盛德 大连海洋大学理学院, 大连 116023  
李晋 盘锦海洋与水产研究所, 盘锦 124010  
陶韦 辽宁省高校近岸海洋环境科学与技术重点实验室, 大连 116023  
李璐瑶 农业部海洋水产增养殖学重点开放实验室, 大连 116023  
马悦欣 农业部海洋水产增养殖学重点开放实验室, 大连 116023 mayuexin@dlou.edu.cn 
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摘要:
为了解翅碱蓬植被对盐沼沉积物微生物的影响,于2013年7月、8月、9月和11月对双台河口裸滩和翅碱蓬植被沉积物(10-15 cm)微生物生物量碳(MBC)、微生物生物量氮(MBN)、16S rRNA基因丰度、潜在硝化速率、β-氨氧化细菌(β-AOB)丰度及群落进行了调查。结果表明,不同采样日期裸滩沉积物MBC、翅碱蓬沉积物β-AOB amoA丰度和两种生境潜在硝化速率没有显著差异;而翅碱蓬沉积物MBC、裸滩沉积物β-AOB amoA丰度、两种生境MBN和16S rRNA基因丰度呈现时间波动。当所有采样日期的数据结合分析时,翅碱蓬植被显著影响沉积物MBC、MBN、细菌16S rRNA基因丰度、潜在硝化速率和β-AOB amoA丰度。从裸滩和翅碱蓬沉积物获得的β-AOB序列属于NitrosospiraNitrosomonas,翅碱蓬植被对β-AOB群落结构和多样性均有一定的影响。研究结果有助于了解翅碱蓬湿地中微生物的作用,为盐沼生境的生态修复技术提供参考。
Abstract:
The goal of this study was to examine the impacts of sedimentary colonization by Suaeda heteroptera on benthic microbial biomass carbon (MBC), microbial biomass nitrogen (MBN), 16S rRNA gene abundance, potential nitrification rate, and the richness and community structure of β-ammonia-oxidizing bacteria (β-AOB) in a salt marsh located in Shuangtai, China. Sediment samples (10-15 cm) were collected from a site colonized by S. heteroptera and from an adjacent unvegetated mudflat in July, August, September, and November of 2013. No significant differences in MBC were detected in unvegetated sediment among the various sampling dates, whereas MBC in vegetated sediment increased significantly in August and September compared with July (P ﹤ 0.05); overall, MBC in vegetated sediment was significantly higher than in unvegetated sediment (P ﹤ 0.05). A similar temporal trend was detected for MBN in the two sites, with higher values recorded in August than in July and November (P ﹤ 0.05); in addition, MBN in vegetated sediment increased significantly in July, August, and September compared with MBN in unvegetated sediment (P ﹤ 0.05). Bacterial 16S rRNA gene abundance, measured using a real-time quantitative PCR technique, was significantly higher in both habitats in July, August, and September than in November (P ﹤ 0.05), and was significantly higher in vegetated sediment in August, September, and November than in unvegetated sediment (P ﹤ 0.05). No significant differences in potential nitrification rates were detected within the two habitats for any of the sampling dates, but potential nitrification rate increased significantly in vegetated sediment in August and September compared with unvegetated sediment (P ﹤ 0.05). The abundance of β-AOB ammonia monooxygenase subunit A gene (amoA) in unvegetated sediment was significantly higher in August than in September and November (P ﹤ 0.05), but no difference was detected in the abundance of β-AOB amoA in vegetated sediment among the different sampling dates. However, β-AOB amoA abundance in vegetated sediment was significantly higher than in unvegetated sediment at all sampling dates (P ﹤ 0.05), with the exception of September. When data from all sampling dates were combined, MBN, 16S rRNA gene abundance, potential nitrification rate and abundance of β-AOB amoA were significantly higher in vegetated sediment than in unvegetated sediment (P ﹤ 0.05). The community structure of β-AOB was analyzed using the denaturing gradient gel electrophoresis (DGGE) technique. Sequences affiliated with species of Nitrosospira and Nitrosomonas were recovered from the two habitats. Colonization by S. heteroptera influenced the community structure and diversity of β-AOB. These results add to our understanding of the role microorganisms play in sediment dominated by S. heteroptera and provide a reference for the ecological restoration of salt marshes.
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